Construction and analysis of cDNA libraries from the antennae of Batocera horsfieldi and expression pattern of putative odorant binding proteins.
Identifieur interne : 002304 ( Main/Exploration ); précédent : 002303; suivant : 002305Construction and analysis of cDNA libraries from the antennae of Batocera horsfieldi and expression pattern of putative odorant binding proteins.
Auteurs : Hui Li [République populaire de Chine] ; Aijun Zhang [États-Unis] ; Li-Zhen Chen [République populaire de Chine] ; Guoan Zhang [République populaire de Chine] ; Man-Qun Wang [République populaire de Chine]Source :
- Journal of insect science (Online) [ 1536-2442 ] ; 2014.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), Animaux (MeSH), Antennes des arthropodes (métabolisme), Banque de gènes (MeSH), Coléoptères (anatomie et histologie), Coléoptères (physiologie), Données de séquences moléculaires (MeSH), Femelle (MeSH), Liaison aux protéines (MeSH), Mâle (MeSH), Réaction de polymérisation en chaine en temps réel (MeSH), Récepteurs olfactifs (génétique), Récepteurs olfactifs (métabolisme), Régulation de l'expression des gènes (MeSH), Séquence d'acides aminés (MeSH), Transcriptome (MeSH), Étiquettes de séquences exprimées (MeSH).
- MESH :
- anatomie et histologie : Coléoptères.
- génétique : ADN complémentaire, Récepteurs olfactifs.
- métabolisme : Antennes des arthropodes, Récepteurs olfactifs.
- physiologie : Coléoptères.
- Animaux, Banque de gènes, Données de séquences moléculaires, Femelle, Liaison aux protéines, Mâle, Réaction de polymérisation en chaine en temps réel, Régulation de l'expression des gènes, Séquence d'acides aminés, Transcriptome, Étiquettes de séquences exprimées.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Animals (MeSH), Arthropod Antennae (metabolism), Coleoptera (anatomy & histology), Coleoptera (physiology), DNA, Complementary (genetics), Expressed Sequence Tags (MeSH), Female (MeSH), Gene Expression Regulation (MeSH), Gene Library (MeSH), Male (MeSH), Molecular Sequence Data (MeSH), Protein Binding (MeSH), Real-Time Polymerase Chain Reaction (MeSH), Receptors, Odorant (genetics), Receptors, Odorant (metabolism), Transcriptome (MeSH).
- MESH :
- chemical , genetics : DNA, Complementary, Receptors, Odorant.
- anatomy & histology : Coleoptera.
- metabolism : Arthropod Antennae, Receptors, Odorant.
- physiology : Coleoptera.
- Amino Acid Sequence, Animals, Expressed Sequence Tags, Female, Gene Expression Regulation, Gene Library, Male, Molecular Sequence Data, Protein Binding, Real-Time Polymerase Chain Reaction, Transcriptome.
Abstract
A high-quality cDNA library was constructed from female and male antenna of the longhorned beetle, Batocera horsfieldi (Hope) (Coleoptera: Cerambycidae), a serious pest of Populus (Salicales: Salicaceae). The titer was approximately 2.37 × 106 pfu/mL, and this complies with the test requirement. From the libraries, 692 clones were selected randomly, sequenced, and further analyzed, and the recombinational efficiency reached 93.85%. By alignment and cluster analysis, we identified four odorant binding proteins, two pheromone-binding proteins (have the characteristic six conserved cysteine residues), four Minus-C odorant binding proteins (lost two conserved cysteines), and three chemosensory proteins. In this study, we describe the identification and characterization of four new cDNAs that encode Minus-C odorant binding proteins (Minus-C OBPs) from B. horsfieldi antennal cDNA libraries. Our investigation focused on the expression pattern of the Minus-C OBP genes in various tissues in both sexes at different developmental stages, using reverse transcription PCR (RT-PCR) and realtime PCR (qPCR) strategies. Minus-C OBP1, 2, and 3 were expressed in all tested tissues, with the exception of the head (without antenna, labial palps, and maxillary palps). Minus-C OBP4 was expressed in the antenna, legs, and abdomen, but not in the labial palps, maxillary palps, or head. The qPCR results revealed MinusC OBPs were expressed in the antenna throughout the adult life, and that the transcript levels of these genes depended on the sex, age, and mating status of adults.
DOI: 10.1093/jis/14.1.57
PubMed: 25373204
PubMed Central: PMC4207511
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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The titer was approximately 2.37 × 106 pfu/mL, and this complies with the test requirement. From the libraries, 692 clones were selected randomly, sequenced, and further analyzed, and the recombinational efficiency reached 93.85%. By alignment and cluster analysis, we identified four odorant binding proteins, two pheromone-binding proteins (have the characteristic six conserved cysteine residues), four Minus-C odorant binding proteins (lost two conserved cysteines), and three chemosensory proteins. In this study, we describe the identification and characterization of four new cDNAs that encode Minus-C odorant binding proteins (Minus-C OBPs) from B. horsfieldi antennal cDNA libraries. Our investigation focused on the expression pattern of the Minus-C OBP genes in various tissues in both sexes at different developmental stages, using reverse transcription PCR (RT-PCR) and realtime PCR (qPCR) strategies. Minus-C OBP1, 2, and 3 were expressed in all tested tissues, with the exception of the head (without antenna, labial palps, and maxillary palps). Minus-C OBP4 was expressed in the antenna, legs, and abdomen, but not in the labial palps, maxillary palps, or head. The qPCR results revealed MinusC OBPs were expressed in the antenna throughout the adult life, and that the transcript levels of these genes depended on the sex, age, and mating status of adults. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25373204</PMID><DateCompleted><Year>2015</Year><Month>06</Month><Day>23</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1536-2442</ISSN><JournalIssue CitedMedium="Internet"><Volume>14</Volume><PubDate><Year>2014</Year><Month>Apr</Month><Day>19</Day></PubDate></JournalIssue><Title>Journal of insect science (Online)</Title><ISOAbbreviation>J Insect Sci</ISOAbbreviation></Journal><ArticleTitle>Construction and analysis of cDNA libraries from the antennae of Batocera horsfieldi and expression pattern of putative odorant binding proteins.</ArticleTitle><Pagination><MedlinePgn>57</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/jis/14.1.57</ELocationID><ELocationID EIdType="pii" ValidYN="Y">57</ELocationID><Abstract><AbstractText>A high-quality cDNA library was constructed from female and male antenna of the longhorned beetle, Batocera horsfieldi (Hope) (Coleoptera: Cerambycidae), a serious pest of Populus (Salicales: Salicaceae). The titer was approximately 2.37 × 106 pfu/mL, and this complies with the test requirement. From the libraries, 692 clones were selected randomly, sequenced, and further analyzed, and the recombinational efficiency reached 93.85%. By alignment and cluster analysis, we identified four odorant binding proteins, two pheromone-binding proteins (have the characteristic six conserved cysteine residues), four Minus-C odorant binding proteins (lost two conserved cysteines), and three chemosensory proteins. In this study, we describe the identification and characterization of four new cDNAs that encode Minus-C odorant binding proteins (Minus-C OBPs) from B. horsfieldi antennal cDNA libraries. Our investigation focused on the expression pattern of the Minus-C OBP genes in various tissues in both sexes at different developmental stages, using reverse transcription PCR (RT-PCR) and realtime PCR (qPCR) strategies. Minus-C OBP1, 2, and 3 were expressed in all tested tissues, with the exception of the head (without antenna, labial palps, and maxillary palps). Minus-C OBP4 was expressed in the antenna, legs, and abdomen, but not in the labial palps, maxillary palps, or head. The qPCR results revealed MinusC OBPs were expressed in the antenna throughout the adult life, and that the transcript levels of these genes depended on the sex, age, and mating status of adults. </AbstractText><CopyrightInformation>This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Hui</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, P. R. China Henan University of Technology, Zhengzhou 450001, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Aijun</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Invasive Insect Biocontrol and Behavior Laboratory, USDA-ARS-Plant Sciences Institute, Beltsville, MD 20705- 2350, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Li-Zhen</ForeName><Initials>LZ</Initials><AffiliationInfo><Affiliation>Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Guoan</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Man-Qun</ForeName><Initials>MQ</Initials><AffiliationInfo><Affiliation>Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, P. R. China mqwang@mail.hzau.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>04</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Insect Sci</MedlineTA><NlmUniqueID>101096396</NlmUniqueID><ISSNLinking>1536-2442</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018035">Receptors, Odorant</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D057506" MajorTopicYN="N">Arthropod Antennae</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001517" MajorTopicYN="N">Coleoptera</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020224" MajorTopicYN="N">Expressed Sequence Tags</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005786" MajorTopicYN="N">Gene Expression Regulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015723" MajorTopicYN="Y">Gene Library</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018035" MajorTopicYN="N">Receptors, Odorant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="N">Transcriptome</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Antennal cDNA library</Keyword><Keyword MajorTopicYN="N">Minus-C OBPs</Keyword><Keyword MajorTopicYN="N">RT-PCR</Keyword><Keyword MajorTopicYN="N">qPCR</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>11</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>11</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>6</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25373204</ArticleId><ArticleId IdType="pii">14.1.57</ArticleId><ArticleId IdType="doi">10.1093/jis/14.1.57</ArticleId><ArticleId IdType="pmc">PMC4207511</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Gene. 1997 Nov 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